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Another drawback of the buccal preparation is the fixed dose of 30 mg 126, 127 clomid 100 mg RNA was prepared using the Qiagen RNeasy kit Crawley, UK and converted to cDNA with the High Capacity cDNA reverse transcription kit, followed by quantitative real time PCR qRT PCR using the TaqMan Gene Expression Assays, listed in supplementary Materials and Methods, Table 1 all from Thermofisher Scientific, Paisley, UK