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Using reverse transcribed cDNA, all PCRs were performed in accordance with the following protocol 5 minutes of denaturation at 95 C, 30 cycles of a 30 second denaturation step at 95 C, 1 minute annealing at 64 C, 30 second elongation at 72 C, and a final elongation step for 5 minutes at 72 C priligy kaufen Aetna Health, Inc